Interleukin 11 (IL11) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL11 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:15.6-1000pg/ml
Sensitivity:5.9pg/ml
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Kit Components:*026072A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*026072B: Standard, 2x1 vial026072C: Standard Diluent, 1x10ml or 1x20ml *026072D: Detection Reagent A (green), 1x60ul or 1x120ul*026072E: Detection Reagent B (red), 1x60ul or 1x120ul026072F: Assay Diluent A, 1x6ml or 1x12ml026072G: Assay Diluent B, 1x6ml or 1x12ml026072H: TMB Substrate, 1x4.5ml or 1x9ml*026072J: Positive Control, 162.8pg/ml, 1x1vial026072K: Stop Solution, 1x3ml or 1x6ml026072L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution:The Stop Solution (026072K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *026072A, *026072B, *026072D, *026072E and *026072J at -20°C. After reconstitution of *026072J, store at 4°C and use within 5 days.Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 026072J: Positive Control:Intended Use:The control vials are from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain rabbit IL11 in rat serum (lyophilized powder). Note: The rat based serum used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).
Expected Value:162.8pg/ml
Usage:Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
