Insulin Like Growth Factor Binding Protein 4 (IGFBP4) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGFBP4 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:0.156-10ng/ml
Sensitivity:0.063ng/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*025883A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*025883B: Standard, 2x1 vial025883C: Standard Diluent, 1x10ml or 1x20ml*025883D: Detection Reagent A (green), 1x60ul or 1x120ul*025883E: Detection Reagent B (red), 1x60ul or 1x120ul025883F: Assay Diluent A, 1x6ml or 1x12ml025883G: Assay Diluent B, 1x6ml or 1x12ml025883H: TMB Substrate, 1x4.5ml or 1x9ml*025883J: Positive Control, 1.83ng/ml, 1x1vial 025883K: Stop Solution, 1x3ml or 1x6ml025883L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution:The Stop Solution (025883K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *025883A, *025883B, *025883D, *025883E and *025883J at -20°C. After reconstitution of *025883J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 025883J Positive Control:Intended Use:The control vials are from rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain rat IGFBP4 in rat serum (lyophilized powder). Note: The rat based material used in this product is from healthy rats of SPF standard (specific pathogen-free animals).
Expected Values:1.83ng/mlThe concentration of the control was determined by subtracting two standard deviations to the mean optical density value of twenty QC sample replicates and calculating the corresponding concentration.
Usage:Disslove with 150ul standard dilutent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
