Beta-Endorphin (bEP) BioAssay™ ELISA Kit (Human) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of bEP in human serum, plasma, cerebrospinal fluid and other biological fluids.
Detection Range:12.35-1000pg/ml
Sensitivity:4.55pg/ml
Intra-Assay CV:< 10%
Inter-Assay CV:< 12%
Kit Components:*023688A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*023688B: Standard, 2x1 vial023688C: Standard Diluent, 1x10ml or 1x20ml *023688D: Detection Reagent A (green), 1x1 vial*023688E: Detection Reagent B (red), 1x60ul or 1x120ul023688F: Assay Diluent A, 1x6ml or 1x12ml023688G: Assay Diluent B, 1x6ml or 1x12ml023688H: TMB Substrate, 1x4.5ml or 1x9ml*023688J: Positive Control, 398.2pg/ml, 1x1vial023688K: Stop Solution, 1x3ml or 1x6ml023688L: Wash Buffer, 30X, 1x10ml or 1x20ml023688M: Detection Reagent A Diluent, 1x300ul
Precaution:The Stop Solution (023688K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *023688A, *023688B, *023688D and *023688E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards;2. Add 50ul standard or sample to each well. Then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
5. Aspirate and wash 5 times
6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C
7. Add 50ul Stop Solution. Read at 450nm immediately.
