The Rabbit Collagen Type 1 Alpha 2 (COL1a2) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1a2 in rabbit serum, plasma and other biological fluids.
Detection Range:0.312-20ng/ml
Sensitivity:0.104ng/ml
Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL1a2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for COL1a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL1a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL1a2 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components:*024268A: Microtiter Plate, 96-well strip plate. Pre-coated, ready to use*024268B: Standard, 2x1vial 024268C: Standard Diluent, 1x20ml*024268D: Detection Reagent A (green), 1x120ul *024268E: Detection Reagent B (red), 1x120ul 024268F: Assay Diluent A, 2x, 1x6ml024268G: Assay Diluent B, 2x, 1x6ml024268H: TMB Substrate, 1x9ml024268K: Stop Solution, 1x6ml024268L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (024268K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *024268A, *024268B, *024268D and *024268E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap
