The Human Matrix Metalloproteinase 7 (MMP7) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MMP7 in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to human MMP7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for human MMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MMP7 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Detection Range:0.312-20ng/ml
Sensitivity:≤0.115ng/ml
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Kit Components:*026748A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use*026748B: Standard, 2x1vial 026748C: Standard Diluent, 1x20ml*026748D: Detection Reagent A, 1x120ul *026748E: Detection Reagent B, 1x120ul 026748F: Assay Diluent A (2x),1x6ml026748G: Assay Diluent B (2x), 1x6ml026748H: TMB Substrate, 1x9ml*026748J: Positive Control (5.48ng/ml), 1x1vial026748K: Stop Solution, 1x6ml026748L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (026748K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *026748A, *026748B, *026748D, *026748E and *026748J at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
