Actin Alpha 1, Skeletal Muscle (ACTa1) BioAssay™ ELISA Kit (Multi) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACTa1 in human, mouse, rat, rabbit, bovine and porcine tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The sequence of ACTa1 recognized by this kit has 100% homology with mouse, rat, rabbit, bovine and porcine.
Precaution:The Stop Solution (023090K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *023090A, *023090B, *023090D and *023090E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
