Apolipoprotein E (APOE) BioAssay™ ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of APOE in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:15.6-1000ng/ml
Sensitivity:6.9ng/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*023530A: Microtiter Plate, 1x48 wells or 1x96 wells*023530B: Standard, 2x1vial*023530C: Detection Reagent A (green), 1x60ul or 1x120ul*023530D: Detection Reagent B (red), 1x60ul or 1x120ul023530E: TMB Substrate, 1x4.5ml or 1x9ml023530F: Wash Buffer (30X), 1x10ml or 1x20ml023530G: Standard Diluent, 1x10ml or 1x20ml023530H: Assay Diluent A, 1x6ml or 1x12ml023530J: Assay Diluent B , 1x6ml or 1x12ml023530K: Stop Solution, 1x3ml or 1x6ml*023530L: Positive Control, 134.7ng/ml, 1x1vial
Precaution:The Stop Solution (023530K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *023530A, *023530B, *023530C, *023530D and *023530L at -20°C. After reconstitution of *023530L, store at 4°C and use within 5 days Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 023530L - Positive Control:Intended Use:The control vials are from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain mouse APOE in mouse serum (lyophilized powder). Note: The mouse based serum used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).
Expected Value:134.7ng/ml
Usage:Dissolve with 150ul standard diluent and let stand for 10min. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
