Cross Linked C-Telopeptide of Type II Collagen (CTXII) BioAssay™ ELISA Kit (Human) is a competitive inhibition immunoassay for the in vitro quantitative measurement of CTXII in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:123.5-10,000pg/ml
Sensitivity:46.3pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*024468A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*024468B: Standard, 2x1 vial024468C: Standard Diluent, 1x20ml*024468D: Detection Reagent A (green), 1x1vial*024468E: Detection Reagent B (red), 1x120ul024468F: Assay Diluent A, 1x12ml024468G: Assay Diluent B, 1x12ml024468H: TMB Substrate, 1x9ml024468K: Stop Solution, 1x6ml024468L: Wash Buffer, 30X, 1x20ml024468M: Detection Reagent A Diluent, 1x300ul
Precaution:The Stop Solution (024468K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *024468A, *024468B, *024468D and *024468E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
5. Aspirate and wash 5 times
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
