Secretory Leukocyte Peptidase Inhibitor (SLPI) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SLPI in human serum, plasma and other biological fluids.
Detection Range:62.5-4000pg/ml
Sensitivity:29.2pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*027994A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use*027994B: Standard, 2x1vial 027994C: Standard Diluent, 1x20ml*027994D: Detection Reagent A (green), 1x120ul *027994E: Detection Reagent B (red), 1x120ul 027994F: Assay Diluent A, 1x12ml027994G: Assay Diluent B, 1x12ml027994H: TMB Substrate, 1x9ml027994K: Stop Solution, 1x6ml027994L: Wash Buffer, 30X, 1x20ml
Storage and Stability:Store *027994A, *027994B, *027994D and *027994E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
