Intended Use:Cross Linked N-Telopeptide of Type I Collagen (NTXI) BioAssay™ ELISA Kit (Rat) is a competitive inhibition immunoassay for in vitro quantitative measurement of NTXI in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:2.47-200ng/ml
Sensitivity:0.92ng/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*024474A: Microtiter Strips, 1x96 wells*024474B: Standard, 2x1 vial024474C: Standard Diluent, 1x20ml*024474D: Detection Reagent A (green), 1x120ul*024474E: Detection Reagent B (red), 1x120ul024474F: Assay Diluent A, 1x12ml024474G: Assay Diluent B, 1x12ml024474H: TMB Substrate, 1x9ml024474K: Stop Solution, 1x6ml024474L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (024474K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *024474A, *024474B, *024474D and *024474E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
5. Aspirate and wash 5 times
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50ul Stop Solution. Read at 450nm immediately.
