Androgen Receptor (AR) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of AR in human tissue homogenates, cell lysates and other biological fluids.
Detection Range:0.312-20ng/ml
Sensitivity:0.128ng/ml
Intra-Assay CV:< 10%
Inter-Assay CV:< 12%
Kit Components:*023351A: Microtiter Strips, 1x96 wells*023351B:. Standard, 2x1 vial, 0.05% Proclin-300023351C: Standard Diluent, 1x20ml, 2% BSA, 0.02% sodium azide in Tris-buffered saline*023351D: Detection Reagent A, 1x120ul, 50% glycerol*023351E: Detection Reagent B, 1x120ul, 50% glycerol023351F: Assay Diluent A, 1x12ml, TBS with 1% BSA and 0.01% sodium azide023351G: Assay Diluent B, 1x12ml, TBS with 1% BSA and 0.01% sodium azide023351H: TMB Substrate, 1x9ml, 0.05% 3,3’,5,5’ tetramethylbenzidine023351J: Positive Control, 1x1vial023351K: Stop Solution, 1x6ml, 1M H2SO4023351L: Wash Buffer, 30X Tris-buffered saline, 1x20ml
Precaution:The Stop Solution (023351K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *023351A, *023351B, *023351D and *023351E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
