Vitronectin (VTN) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of VTN in human serum, plasma and other biological fluids.
Detection Range:7.8-500pg/ml
Sensitivity: 3.2pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*028904A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *028904B: Standard, 2x1 vial 028904C: Standard Diluent, 1x20ml *028904D: Detection Reagent A (green), 1x120ul *028904E: Detection Reagent B (red), 1x120ul 028904F: Assay Diluent A, 1x12ml 028904G: Assay Diluent B, 1x12ml 028904H: TMB Substrate, 1x9ml 028904K: Stop Solution, 1x6ml 028904L: Wash Buffer, 30X, 1x20ml
Precaution: The Stop Solution (028904K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *028904A, *028904B, *028904D and *028904E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing
Assay Procedure Summary:1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to eac well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour a 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reag B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubat 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
