85-1723-51　Carbonic Anhydrase II (CA2) BioAssay™ ELISA Kit (Human) 96Tests　023901

特徴

• Carbonic Anhydrase II (CA2) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CA2 in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
• Detection Range:3.12-200ng/ml
• Sensitivity:1.34ng/ml
• Intra-Assay:CV≤10%
• Inter-Assay:CV≤12%
• Kit Components:*023901A: Microtiter Plate, 1x96wells, Pre-coated, ready to use*023901B: Standard, 2x1vial 023901C: Standard Diluent, 1x20ml*023901D: Detection Reagent A, 1x120ul *023901E: Detection Reagent B, 1x120ul 023901F: Assay Diluent A, 2x, 1x6ml023901G: Assay Diluent B, 2x, 1x6ml023901H: TMB Substrate, 1x9ml*023901J: Positive Control, 18.92ng/ml, 1x1vial023901K: Stop Solution, 1x6ml023901L: Wash Buffer, 30X, 1x20ml
• Precaution:The Stop Solution (023594K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
• Storage and Stability:Store *023901A, *023901B, *023901D, *023901E and 023901J at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Assay Procedure Summary:1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
• 3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 5. Aspirate and wash 5 times
• 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C. 7. Add 50ul Stop Solution. Read at 450nm immediately.