Coagulation Factor IX (F9) BioAssay™ ELISA Kit (Mouse) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of mouse F9 in plasma.
Detection Range:0.312-20ng/ml
Sensitivity:0.136ng/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*024225A: Microtiter Strip, 1x96 wells, Pre-coated; ready to use*024225B: Standard, 2x1vial 024225C: Standard Diluent, 1x20ml*024225D: Detection Reagent A (green), 1x120ul *024225E: Detection Reagent B (red), 1x120ul 024225F: Assay Diluent A, 1x12ml024225G: Assay Diluent B, 1x12ml024225H: TMB Substrate, 1x9ml024225K: Stop Solution, 1x6ml024225L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (024225K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *024225A, *024225B, *024225D and *024225E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
