A highly sensitive tetrazolium reagent (light red) that produces a water-soluble formazan (dark red color) in NADH and cell proliferation assays due to cleavage by mitochondrial dehydrogenase.
WST-1 formazan: l max = 438nm; e= 3.7 x 10e4
Atmosphere:Inert gas
Melting Point:>270°C (dec.)
Solubility:DMSO (Slightly), Methanol (Slightly), Water (Slightly)
Method for Determining Identity:Proton NMR (DMSO-d6) Spectroscopic and Mass Spectrometric Analysis
TLC Conditions:C18; Water: Methanol: Acetic Acid=6:4:0.1; Visualized with UV and KMnO4; Single spot, Rf=0.55
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic and light sensitive. Store under inert atmosphere.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
