A red Cy3-based biarsenical fluorescent probe targeted to a complementary binding peptide. AsCy3 has good photostability, low pH sensitivity, high absorbance, and good quantum yield. This new probe provides a FRET partner to biarsenical dye FlAsH, making this discovery an important step toward a whole toolkit of colored probes directed to different small peptide motifs.
TLC Conditions:SiO2; Dichloromethane : Methanol : Acetic Acid = 8 : 2 : 0.2; Visualized with UV, KMnO4, and Naked Eye; Single Spot, Rf = 0.60.1H NMR and MS conform to structure.
Solubility:DMSO (Slightly)
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Amber Vial, under Inert Atmosphere. For maximum recovery of product, centrifuge the original vial prior to removing the cap.