L-Phenylalanine is one of the twenty common amino acids and an important precursor for several key signal molecules such as dopamine, norepinephrine, epinephrine, and the skin pigment melanin. It is found naturally in the breast milk of mammals, and used as nutritional supplements in food and drink products. The genetic disorder phenylketonuria is the inability to metabolize phenylalanine. Individuals with this disorder are known as "phenylketonurics". Individuals who cannot metabolize phenylalanine must monitor their intake of protein to control the buildup of phenylalanine.
L-Phenylalanine Assay Kit provides a convenient fluorometric means to measure L-phenylalanine in biological samples. In the assay, L-phenylalanine is oxidized by phenylalanine dehydrogenase, producing NADH, which reduces a fluorescent dye to a highly fluorescent product. The resulting fluorescence intensity (lexc/em=530/595nm) is linear to the L-phenylalanine concentration in the sample.
Key Features:Safe: Non-radioactive assay
Sensitive and accurate: Linear detection range of 2-300uM L-phenylalanine
Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Applications:Determination of L-phenylalanine in serum, urine and other biological samples.
Kit Components:P4060-05G1: Assay Buffer, 1x10ml *P4060-05G2: Enzyme A, 1x1vialP4060-05G3: Enzyme B, 1x120ulP4060-05G4: Standard, 1x120ulP4060-05G5: NAD Solution, 1x1ml P4060-05G6: Probe, 1x750ul
Storage and Stability:Store all components at -20°C. Stable for 6 months after receipt. Store reconstituted *P4060-05G2 at 4ºC Do Not Freeze and use within 1 month. For maximum recovery of product, centrifuge the original vials prior to removing the cap.
