Lactate Dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565nm. The intensity of the purple color formed is directly proportional to the enzyme activity.
Key Features:High sensitivity and wide linear range: Use 3ul serum or plasma sample. The detection limit is 2IU/L, linear up to 200IU/L.
Homogeneous and simple procedure: Simple “mix-and-measure” procedure allows reliable quantitation of LDH activity within 30 minutes.
Robust and amenable to HTS: All reagents are compatible with high throughput liquid handling instruments.
Applications:Direct Assays: LDH activity in serum, plasma and other sources
Characterization and Quality Control for LDH production
Drug Discovery: Screen and evaluation of LDH modulators.
Kit Components: (100 tests in 96-well plates):L1013-95A: Substrate Buffer, 1x20mlL1013-95B: Diaphorase, 1x120ulL1013-95C: NAD Solution, 1x1mlL1013-95D: Calibrator, 1x1.5mlL1013-95E: MTT Solution, 1x1.5ml
Storage and Stability:Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
