Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. Lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 56 nm, is proportionate to the lactate concentration in the sample.
Applications:Direct Assays: Lactate in serum, plasma, and cell media samples.
Key Features:Sensitive and accurateDetection limit:96-well plate assay: 0.05mM and linearity up to 2mM L-Lactate. For cell culture samples containing phenol red: 0.1mM and linearity up to 1mM L-Lactate in 96-well plate assay.
Convenient: The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 minutes. Room temperature assay. No 37°C heater is needed.
High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Kit Components: (100 tests in 96-well plates):L1011-04A1: Assay Buffer, 1x10ml L1011-04A2: NAD Solution, 1x1mlL1011-04A3: Enzyme A, 1x120ul L1011-04A4: Enzyme B, 1x120ul L1011-04A5: MTT Solution, 1x1.5mlL1011-04A6: Standard, 1x1ml Supplied in 20mM L-Lactate
Storage and Stability:Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Precautions:Reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.
