Glyoxalase I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-Slactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer.
Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. Glyoxalase I Assay Kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, Slactoylglutathione, by measuring the change in absorbance at 240nm.
Applications:Direct Assays: GLO-1 activity in enzyme preparations or biological samples
Drug Discovery/Pharmacology: Effects of drugs on GLO-1 activity.
Key Features:
Sensitive and accurate. Detection limit 4 U/L GLO-1 activity.
Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate. Can be readily automated as a high-throughput assay for thousands of samples per day.
Storage and Stability:Store *G8235-30E3 at RT. Store other components at -20º°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
