Glutamine is an amino acid synthesized in the muscle that plays major roles in protein synthesis, acid-base balance, anabolic processes and is utilized for cellular energy and as a carbon source. It is used in treatment of injury, trauma, burns, and also as a supplement for muscle growth and post-surgery healing.
Simple, direct and automation-ready procedures for measuring glutamine concentration are very desirable. This Glutamine Assay Kit is based on hydrolysis of glutamine to glutamate and colorimetric determination of the product. The intensity of the product color, measured at 565nm, is proportional to the glutamine concentration in the sample.
Key Features:Sensitive and accurate: Use 20ul sample. Linear detection range of 0.023-2mM glutamine in 96-well plate assay
Convenient: The procedure involves adding a single working reagent, incubation for 40 minutes at RT, adding a stop reagent and reading the optical density. No 37°C heater is needed
High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Applications:Direct Assays: Glutamine in serum, plasma, urine, tissue extracts and cell culture samples. Drug Discovery/Pharmacology: Effects of drugs on glutamine metabolism.
Storage and Stability:Store all components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials prior to removing the cap.
