b-Glucosidase is a glucosidase enzyme which acts upon beta1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). b-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a b-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a b-glucosidase isoform (lysosomal glucocerebrosidase) has been related to Gaucher's disease and Parkinson's disease. This b-Glucosidase Assay Kit is designed to measure b-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-b-D-glucopyranoside that is hydrolyzed specifically by b-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
Key Features:High sensitivity and wide linear range. Use 20ul sample. The detection limit is 2U/L, linear up to 250U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of b-glucosidase activity within 20 minutes. Robust and amenable to high throughput analysis.
Applications:Direct Assays: b-glucosidase activity in biological samples. Characterization and Quality Control for b-glucosidase production. Drug Discovery: high-throughput screen for b-glucosidase modulators.
Unit Definition:One unit of enzyme catalyzes the hydrolysis of 1mmole of substrate per min at pH 7.0.
