ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation
ATPase/GTPase Assay Kit offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 minutes at RT. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600-660nm).
Key Features:High Sensitivity: Detection of 0.007U/L ATPase or GTPase activity
Fast and Convenient: Single reagent, homogeneous “mix-and-measure” assay allows quantitation of enzyme activity within 30 minutes
Robust and Amenable to HTS: Detection at 620nm greatly reduces potential interference by colored compounds. Z’ factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.
Applications:Determination of ATPase and GTPase activity
Drug Discovery: High-throughput screen for ATPase or GTPase inhibitors.
Storage and Stability:Store components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Precautions: A3999-23A: Reagent contains 0.27M H2SO4. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.
