Alpha Amylase acts upon large polysaccharides at the alpha-1,4-glucoside bonds. The hydrolytic products have alpha configuration. The activity is present in all living organisms, however the enzymes vary remarkably even from tissue to tissue within a single species.
Source:Amylase, alpha, from porcine pancreas.
Synonyms:1,4-alpha-D-Glucan-glucanohydrolase; amy2; PPA; PA
Appearance:Supplied as a lyophilized powder from 150mM sodium chloride, 3mM calcium chloride. Reconstitute with Tris saline buffer.
Activity:~230Units/mg powder @ 37°C
Protein:~0.4mg Protein/mg powder
Specific Activity:~480Units/mg
Unit Definition:One unit will catalyze the hydrolysis of one micromole of 2-chloro-4-mitrophenyl-alpha-D-maltrtrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
Molecular Weight:~54,000
E.C. Number:3.2.1.1
Gene:AMY2
Gene ID:397397
Accession No:P00690
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile tris saline buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
