Mouse interleukin-36 beta [IL-36B; previously IL-1F8, FIL-1h (eta) and IL-1H2] is a member of the IL-1 family of proteins that includes IL-1B, IL-1A, IL-1ra, IL-18, IL-36Ra/IL-1F5, IL-36A/IL-1F6, IL-37/IL-1F7, IL-36g/IL-1F9 and IL-1F10. All family members show a 12 B-stranded B-trefoil configuration, share up to 50% aa sequence identity, and are believed to have arisen from a common ancestral gene. Although two alternatively spliced transcript variants for human IL-36B/IL-1F8 have been described, to date, only one mouse IL-36B/IL-1F8 isoform is known. Mouse IL-36B/IL-1F8 is synthesized as a 183aa protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s). Mouse IL-36B/IL-1F8 shares 61% and 74% aa identity with human IL-36B isoform 2 and rat IL-36B, respectively. IL-36B is agonistic, stimulating release of inflammatory mediators such as IL-6 and IL-8, and cytotoxic peptides such as beta-defensins 2 and 3 that aid in defense against microbial pathogens. The receptor for IL-36 proteins is IL-1 Rrp2, with IL-1 RAcP as a coreceptor. Antagonism of IL-36 proteins by IL-36Ra, which also binds IL-1 Rrp2, has been shown by some investigators. Skin keratinocytes express highest levels of IL-36 proteins and their receptors, followed by epithelia in the esophagus, trachea and bronchae. IL-36B expression is increased in psoriatic skin and may play a role in pathogenesis of psoriasis. IL-36B is also expressed in resting and activated monocytes and B cells, synovial fibroblasts, neurons and glia, and is detectable in plasma and body fluids. IL-36B, along with IL-36A and IL-36G, is up-regulated by IL-1A and TNF-A in keratinocytes, and has been shown to activate NF-kB and MAPK signaling pathways in an IL-1 Rrp2-dependent manner. Full-length recombinant IL-36 proteins appear less active than their endogenous counterparts, but trimming of the N-termini enhances their activity.
Source:Recombinant corresponding to aa31-183 from mouse IL-36 beta, expressed in E.coli.
Molecular Weight:~17.4kD
Biological Activity:Measured by its ability to induce IL-6 secretion by NIH/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 1-6mg/ml.
Endotoxin: ~1EU/1ug (LAL)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in MES, EDTA, sodium chloride, CHAPS, PEG8000, TCEP, BSA. Reconstitute with 10mM Tris-HCl, pH 8.0 to 0.1mg/ml.
