CRISP-4 is one of several CRISPs (cysteine-rich secretory proteins) that contain 16 invariant cysteine residues and show structural homolgy to snake, insect, and lizard venom proteins. These proteins consist of an N-terminal SCP/CAP domain, a hinge region, and a cysteine-rich CRISP domain. CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction. CRISP-4 facilitates the progesterone-induced acrosomal reaction by inhibiting calcium conductance through the TRPM8 channel on sperm. Mouse CRISP-4 is secreted as a 32kD protein in the epididymis (primarily the caput region), vas deferens, and seminal vesicle. It is also associated with spermatozoa from the testis and caudal epididymis as a 26-28kD molecule. Its expression is induced at the onset of puberty in an androgen-dependent fashion. Mouse and rat CRISP-1 are additionally expressed in salivary glands, while CRISP-4 is not. Expression patterns, genomic structure, and sequence conservation indicate that CRISP-4 in mouse and rat are the likely orthologs of human CRISP-1. Mature mouse CRISP-4 shares 90% and 64% aa sequence identity with rat CRISP-4 and human CRISP-1, respectively. Mature mouse CRISP-4 shares 38%-43% aa sequence identity with mouse CRISP-1, -2, and -3.
Source:Recombinant corresponding to aa22-250, Pro22-Lys250, from mouse CRISP-4, fused to 6-His-tag at C-terminal expressed in Chinese Hamster Ovary cell line, CHO.
Molecular Weight:~27.2kD
Biological Activity:Measured by the ability of the immobilized protein to support the adhesion of NTera-2 human testicular embryonic carcinoma cells (ATCC: CRL-1973). The ED50 for this effect is typically 0.3-1.5ug/ml after a 1h incubation at 37 °C.
Endotoxin: ~1EU/1ug (LAL)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with 250ul sterile PBS.
