Preparation of HSV-2 antigens:HSV-2 is propagated in mammalian cells using a protocol optimized for maximum particle yield. At the appropriate time, infected cells are washed in saline then disrupted to release virus particles by freeze thaw and sonication.
Purification and presentation:PBS lysate: Virus particles released from cells are partially purified by low speed centrifugation to remove cell debris. HSV-2 particles are then purified by sucrose density gradients. This process also includes a DNase step to remove cellular DNA and helps to achieve a high purity.
Source:HSV-2, strain G, from a crude lysate.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:0.1% Triton X-100. Tested by passage on permissive cells for 5 days yielding no CPE.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note:No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.
仕様
Size：200ul
Source Antigen：Crude lysate
Grade：Purified
Purity：Purified by sucrose gradient centrifugation then dialyzed.
Form：Supplied as a liquid in PBS, 0.1% Triton X-100.
