Chlamydia trachomatis is propagated to maximum density in McCoy cells under Category 2 safety conditions. Infected cells are carefully washed and disrupted into buffered saline (PBS), subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris. Chlamydia antigen production is scalable from 10ml to several liters and is optimized by the multiplicity of infection and by harvesting time.
Inactivation is by heat treatment and the addition of triton X-100 to render products non-infectious and improve stability. All preparations are tested as no infectious by double passage on McCoy cell under optimal growth conditions.
Source:Chlamydia trachomatis, LGV2 strain 434, from clarified cell lysate.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Heat treatment and 0.05% Triton X-100. Tested inactive by two blind passages on permissive cells showing no signs of inclusion bodies.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note:No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.
仕様
Size：1ml
Source Antigen：Clarified cell lysate
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.05% Triton X-100.
