Heparan K5 Lyase is a recombinant enzyme. Each aliquot contains 100 milli-international units and 100ug total protein (92ug BSA as carrier) lyophilized from solution in 50mM Tris, 50mM NaCl, and 0.1% Triton X100, pH 8.0. The enzyme is feeze-dried in 100ul aliquots from
The unit definition is the activity that releases 1 micromole of delta (4,5) hexuronate per minute at 37°C using an extinction coefficient of 5100 per cm per M at 232nm for the delta (4,5) hexuronate product.
Molecular Weight:~52.3kD
Unit Definition:One unit equals the amount of enzyme that releases 1 micromole of delta (4,5) hexuronate per minute at 37°C using an extinction coefficient of 5100 per cm per M at 232nm for the delta (4,5) hexuronate product.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100MU
Source Antigen:Recombinant, E. coli, strain TOP10, a disabled K-12 strain