85-1585-88　Vicia villosa Lectin (VVA, VVL) (Agarose) 2ml　V2121-94A

特徴

• This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially alpha- or beta-linked terminal N-acetylgalactosamine, especially a single alpha N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the “Tn antigen”). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (a-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin. Agarose bound Vicia Villosa Lectin is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10e7 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. The technique developed to couple lectins to agarose provides a very hydrophilic spacer arm between the protein and the matrix. This ensures maximum expression of the carbohydrate binding activity of the lectin. The linkage is very stable over a range of pH values and, unlike cyanogen bromide linkages, proteins are not leached off the gel by Tris or other routinely used buffers. In addition, residual charges generated during cyanogen bromide conjugation which can produce nonspecific binding are not present on the gel following our coupling procedure.
• Applications:Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Binding Capacity:≥2.5mg of N-acetylglucosaminyl glycoprotein/ml gel
• Inhibiting/Eluting Sugar:200mM N-acetylgalactosamine 3mg lectin/ml gel
• Activity:2x10e7
• Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.