Ulex europaeus agglutinin I is a glycoprotein with a molecular weight of 63,000, although multimeric aggregates have been reported. UEA I has two subunits, one of about 31,000D and another of 32,000D. UEA I binds to many glycoproteins and glycolipids containing a -linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inhibiting/Eluting Sugar:50mM-100mM L-fucose
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2mg
Source Antigen：Ulex europaeus (Furze gorse) seeds
Grade：Molecular Biology Grade
Form：Supplied as a powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.1mM Ca++, 0.08% sodium azide. Reconstitute with 1ml dH2O. Labeled with Biotin.
