85-1585-82　［受注停止］Ulex europaeus (UEA I, Gorze, Furze) (Agarose) 2ml　U1500-15A

特徴

• Ulex europaeus agglutinin I is a glycoprotein with a molecular weight of 63,000, although multimeric aggregates have been reported. UEA I has two subunits, one of about 31,000D and another of 32,000D. UEA I binds to many glycoproteins and glycolipids containing a-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells. Agarose bound Ulex Europaeus Agglutinin I is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10e7 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. The technique developed to couple lectins to agarose provides a very hydrophilic spacer arm between the protein and the matrix. This ensures maximum expression of the carbohydrate binding activity of the lectin. The linkage is very stable over a range of pH values and, unlike cyanogen bromide linkages, proteins are not leached off the gel by Tris or other routinely used buffers. In addition, residual charges generated during cyanogen bromide conjugation which can produce nonspecific binding are not present on the gel following our coupling procedure.
• Applications:Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Binding Capacity:2mg of fucosyl glycoprotein/ml gel
• Inhibiting/Eluting Sugar:50mM-100mM L-fucose
• Storage and Stability:May be stored at 4°C. Do not freeze. Stable for 6 months after receipt For maximum recovery of product, centrifuge the original vial prior to removing the cap.