85-1585-46　Protein A, S. aureus, Cowan Strain (Biotin) 1mg　P9102-04N

特徴

• Protein A, isolated from a special strain of Staphylococcus aureus, is a 41,000D protein capable of binding to the Fc portion of immunoglobulin. This interaction is not species specific, as Protein A has been shown to bind IgG from a variety of species. The binding was thought to be restricted to IgG, but subsequent studies have demonstrated that Protein A can interact with IgA, IgM and subclasses of IgG from different species. Biotinylated Protein A is a useful reagent for detecting or localizing immunoglobulins on cell surfaces or in tissue sections. Used with the appropriate Avidin D conjugate, immunoglobulins can be studied at the fluorescent or electron microscopic level. Biotinylated Protein A can also be employed for the detection of positive clones in monoclonal antibody production by using Avidin D conjugated with peroxidase or other marker enzymes. Used in many systems as a substitute for a biotinylated second antibody. Because Biotinylated Protein A may produce a high background by binding to endogenous immunoglobulin in the tissue, or it may not bind to the species or class of pri-mary antibody employed, we recommend using biotinylated anti-immunoglobulins for routine immunohistochemical staining.
• Solid-phase assay of immunoglobulin reactivity normalized to rabbit IgG:Protein: Reactivity:Human IgG: 100% Bovine, feline, canine, goat, guinea pig, equine, murine, sheep, swine and Rhesus monkey IgG: 50% Rat IgG: 25%Chicken and Hamster IgG: 1%
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.