After iontophoretic injection of PHA-L, the approximate rate of anterograde transport is about 4-6mM/day, with survival periods of over 18 days having been observed. Once transported, the PHA-L is best visualized for light microscopy with antibody to the lectin, followed by an appropriate ABC kit and DAB. Fluorescent staining can also be used for a faster staining procedure.
Source:Red kidney bean
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
