Lotus tetragonolobus lectin is a family of closely related glycoproteins having 2 or 4 subunits of about 28,000D. The isoelectric points differ somewhat but these isolectins probably have similar specificities toward alpha-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are markedly different between these fucose-specific lectins.
Applications:User-Optimized.
Inhibiting/Eluting Sugar:50mM-100mM L-fucose
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5mg
Source Antigen：Lotus tetragonolobus seeds
Grade：Molecular Biology Grade
Purity：2 bands by SDS-PAGE corresponding to the subunits of thelectin
Form：Supplied as a lyophilized powder. Although many buffers can be employed for reconstituting and diluting L3445-10, 10mM HEPES buffered saline, pH 7.5 - 8.5, 0.1mM Ca++ is recommended. L3445-10 may be preserved with 0.04% sodium azide.
