GSL I-B4 isolectin contains only the B subunits and has been reported to be useful as a marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat. Fluorescein labeled GSL I – isolectin B4 is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled GSL I – isolectin B4 has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.
Applications:Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Inhibiting Sugar:500mM galactose or 100mM raffinose
F/P (molar):As reported
Excitation:495-500nm
Emission:514-521nm
Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Grade：Molecular Biology Grade
Form：Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide, 0.1mM Ca++. Labeled with Fluorescein.
