GSL I is a family of glycoproteins with molecular weights of ~114kD. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.
Inhibiting Sugar:500mM galactose or 100mM raffinose
Applications:G8967-93 will agglutinate blood group B glutaraldehyde-fixed human red blood cells at a concentration of 2ug/ml. No agglutination of blood group A, glutaraldehyde-fixed, human red blood cells occurred at concentrations up to 0.1mg/ml.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Grade：Molecular Biology Grade
Purity：Homogeneous by SDS-PAGE
Form：Supplied as a lyophilized powder. Reconstitute in 10mM HEPES, pH 8.5, 0.1mM Ca++. For preserving solutions, stored at 4ºC, 0.08% sodium azide can be used
