85-1585-06　Griffonia simplicifolia GS I (Bandeiraea simplicifolia) (FITC) 2mg　G8967-17A

特徴

• GSL I is a family of glycoproteins with molecular weights of approximately 115,000. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for a-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues. Our GSL I is a mixture of the five isolectins. Binding appears to require divalent cations such as calcium and magnesium. GSL I has been reported to bind several glycoproteins including laminin. Fluorescein labeled Griffonia Simplicifolia Lectin I is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Griffonia Simplicifolia Lectin I has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.
• Applications:Suitable for use in FLISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Inhibiting/Eluting Sugar:200mM galactose/200mM N-acetylgalactosamine mixture
• Excitation:495nm
• Emission:515nm
• F/P (molar):3.7
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.