Fluorescein Maleimide is designed for fluoresceinating proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Fluorescein Maleimide require free thiol groups. In the case of proteins, Fluorescein Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane). For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag, 5’ EndTag or 3’ EndTag nucleic acid labeling systems
This fluorescent label can be directly detected or used as a hapten and detected with our high affinity antibody reagents.
Molecular Weight:498.4
Molecular Formula:C27H18N2O8
Exitation:492nm
Emission:520nm
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
