Erythrina cristagalli lectin is a 54,000D glycoprotein consisting of two different subunits of approximately 28,000 and 26,000D. It has a specificity toward galactose residues and appears to have the highest binding activity toward galactosyl (b -1,4) N-acetylglucosamine
This lectin has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique. Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose
The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin. Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins. ECL has also been reported to be mitogenic to human peripheral T lymphocytes but is not mitogenic toward human peripheral B lymphocytes, mouse splenocytes or mouse thymocytes. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5mg
Source Antigen：Erythrina cristagalli seeds
Grade：Molecular Biology Grade
Form：Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.1mM Ca++, 0.08% sodium azide. Labeled with Biotin.
