This tetrameric lectin consists of 44,000D subunits and does not require divalent cations for binding. Binding appears to be highest for glycoconjugates containing galactosyl (b-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal alpha linked N-acetylgalactosamine can also bind. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inhibiting/Eluting Sugar:100mM lactose
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5mg
Source Antigen：Bauhinia purpurea alba seeds
Grade：Molecular Biology Grade
Purity：Homogeneous by SDS-PAGE
Form：Supplied as a lyophilized powder in ~18% salts. We recommend using 10mM HEPES buffered saline, pH 8.5, 0.1mM Ca++ for reconstitution and diluting. For preserving solutions at 4ºC, 0.08% sodium azide should be used.
