Horseradish Peroxidase Avidin D (HRP Avidin D) is produced by coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled Avidin D, antigens can be localized in histological sections, cytospin preparations, or smears. In addition, sensitive detection systems for a variety of solid-phase assays utilizing microtitration plates or nitrocellulose have been developed using this conjugate. Horseradish Peroxidase Avidin D is offered as a concentrate (5mg/ml) or in a ready-to-use (R.T.U.) stabilized solution (100ml,1ug/ml) in a bottle fitted with a drop dispenser top.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Moles of HRP/Avidin:1.5
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5mg
Source Antigen：Horseradish peroxidase
Form：Supplied as a liquid in 0.05M sodium bicarbonate, pH 8.3, 0.05% thimerosal. Labeled with horseradish peroxidase (HRP).
