Avidin D is isolated from egg white by a specific affinity system which results in a homogeneous, ultrapure avidin with very low nonspecific binding characteristics. Avidin D is recommended for use in solid-phase assays and many histochemical applications when the technique of sequential addition of reagents (“sandwich” technique) is employed.
Applications:Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:Immunohistochemistry: 5-50ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sodium bicarbonate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10mg
Source Antigen：Isolated from egg white
Grade：Highly Purified
Purity：~97% (SDS-PAGE)
Form：Supplied as a powder, no preservatives added. Reconstitute with 0.1M sodium bicarbonate, 0.09% sodium azide.
