Recombinant protein corresponding to 144aa of human Interferon gamma expressed in E.coli is a single, non-glycosylated, polypeptide chain.
Biological Activity:The specific activity as determined in a viral resistance assay is <0.05ng/ml, corresponding to a specific activity of 2.0x10e7 IU/mg.
Dimers and Aggregates:≤1% (SDS-PAGE)
Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences(IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IFN-gamma as a Reference Standard.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
