Human CD27 is a lymphocyte specific member of the tumor necrosis factor receptor family (TNFRSF7) and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the TNF ligand superfamily (TNFSF7). The CD27-CD70 interaction plays an important role in T cell activation.
Reactivity:Recombinant soluble CD27-muIg binds to cell surface CD70 on Raji cells in FACS, and is reactive with recombinant CD70-muCD8 and CD27.
Applications:Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:1. 5x10e5 cultured human Raji cells were washed and pre incubated 5 minutes with 20ul of 300ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of C2379-12F at 10ug/ml.
2. Cells were washed twice and incubated with secondary reagent Streptavidin/R-PE (Cat #S7973-88P), after which they were washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 0.56 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
4. Binding was blocked when reagent was pre incubated with a 5-fold excess (mg/mg) of recombinant soluble CD70-muCD8 (Cat #C2425-01Q).
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
