Wnt-10b (also known as Wnt-12) is a 42-44kD member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mouse Wnt-10b cDNA encodes a 361aa precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). A short isoform is reported that lacks aa 143 - 238 of the precursor (1, 4). Mouse Wnt-10b shares 97-98% aa identity with human, rat, equine, porcine and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5-7). Mouse Wnt-10b deletion produces age-dependent loss of bone mass due to defective production of osteoblasts, while transgenic over-expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5-7). Ectopic expression of Wnt-10b in an obesity and diabetes-prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a mis-sense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10 is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14). As a key regulator, Wnt signaling plays a major role in the process of colon carcinogenesis (15).
Source:Recombinant corresponding to aa1-389 of mouse Wnt-10b, expressed in CHO cells.
Molecular Weight:~40.1kD
Endotoxin Level:≤1EU/1ug (LAL)
Biological Activity:Measured in a cell proliferation/survival assay using IEC-18 rat small intestinal epithelial cell line (ATCC: CRL-1589). The ED50 for this effect is typically 0.9-3.6ug/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, CHO
Grade：Purified
Purity：~85% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS, EDTA, CHAPS. Reconstitute with PBS to 100ug/ml.
