Inorganic Pyrophosphatases are enzymes that catalyze the hydrolysis of pyrophosphate to two phosphate ions (1). This reaction is highly exergonic, and is utilized in many biochemical pathways, such as DNA synthesis (2) and bone formation (3), to render reactions effectively irreversible (4, 5). Likewise, inorganic pyrophosphatases can be coupled to unfavorable biochemical reactions in order to drive these reactions to completion, such as in the synthesis of activated sulfur donor 3’-phosphoadenosine-5’-phosphosulfate (PAPS) (6). Two inorganic pyrophosphatases, i.e. PPA1 and PPA2, are found in humans. PPA1 is highly active on pyrophosphate and is ubiquitously expressed (7).
Source:Recombinant corresponding to aa1-289 from human PPA1, fused with 6-His tag at C-terminal, expressed in E. coli.
Molecular Weight:~33kD
Endotoxin Level:≤1EU/1ug (LAL)
Biological Activity:Measured by its ability to hydrolyze pyrophosphate. The specific activity is >50,000 pmoles/min/ug, as measured under the described conditions.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~85% (SDS-PAGE)
Form：Supplied as a liquid in Tris, sodium chloride, DTT.
