O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1-X-S-X-P-C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C-terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T-acute lymphoblastic leukemias (3). Recently, POGLUT1 has been demonstrated in a phosphatae- coupled glycosyltransferase assay to have hydrolase activity on UDP-Glc (4).
Source:Recombinant corresponding to aa1-388 from human POGLUT, fused with 6-His tag at C-terminal, expressed in CHO cells.
Molecular Weight:~44kD
Endotoxin Level:≤1EU/1ug (LAL)
Biological Activity:Recombinant corresponding to aa1-388 from human POGLUT CHO cell line, with a C-terminal 6-His tag expressed in CHO cells.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a liquid in Tris, sodium chloride.
