Neural cell adhesion molecule 1 is a membrane-bound glycoprotein that plays an important role in nervous system development and function (1, 2). Mature mouse NCAM-1 consists of a 692aa extracellular domain (ECD) with five tandem Ig-like domains and two fibronectin type III domains, an 18aa transmembrane segment, and a 386aa cytoplasmic domain (3). Three major splice variants of NCAM-1 are expressed: the 180kD full length NCAM-180 isoform, the 140kD NCAM-140 isoform which lacks most of the cytoplasmic domain, and the 120kD GPI-anchored NCAM-120 isoform that includes the ECD only. Splicing is tissue specific and developmentally regulated (4-6). Within the ECD, mouse NCAM-1 shares 94% and 95% aa sequence identity with human and rat NCAM-1, respectively. It is expressed on neurons and glial cells, skeletal muscle, and immune NK cells (5, 7-9). NCAM-1 is extensively modified with polysialic acid (PSA) during development, but this addition is decreased in adult tissues (5, 6, 8). Polysialylation of NCAM-1 is retained in the adult hippocampus where it is important for synaptic plasticity and memory formation (10). The PSA moiety also participates in the binding of NCAM-1 to heparan sulfate proteoglycans and NCAM-1 mediated migration of olfactory neurons (11, 12). Proteolytic shedding of NCAM-1 liberates a soluble ECD fragment that can inhibit cortical neurite branching and growth (6). The NCAM-140 isoform is preferentially expressed on NK cells that robustly secrete cytokines upon activation (9, 13). Selective up-regulation of the NCAM-140 isoform in a variety of tumors initiates epithelial-mesenchymal transition (EMT) and promotes tumor cell invasion (14, 15). Finally, NCAM-1 is known to interact with a number of transmembrane and extracellular molecules. NK cell NCAM-1 binds to T cell FGF R1, co-stimulating IL-2 production by T cells (16). NCAM-1 also forms a noncovalent membrane complex with GFRa1, 2 and 4, generating a receptor for GDNF, NTN and PSP, respectively (17). And NCAM-1 is reported to form homophilic trans-interactions, and to interact with L1 CAM in cis, and with HSPGs (agrin and collagen XVIII) in trans. In general, these interactions are involved in cell adhesion, migration, and/or process extension (18).
Source:Recombinant corresponding to aa1-711 from mouse CD56, fused with 6-His tag at C-terminal, expressed in mouse myeloma cell line, NSO cells.
Molecular Weight:~77.4kD
Endotoxin Level:≤1EU/1ug (LAL)
Biological Activity:Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a mouse neuroblastoma cells (ATCC: CCL-131). When 5x104cells/well are added to mouse NCAM-1 coated plates (25ug/ml with 100ul/well), >20% will adhere after 1 hour incubation at 37°C
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NSO
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.
