85-1574-12　11-dCTP (Biotin) 5x200ul　D3187-03K

特徴

• Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications.
• Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine.
• Note:The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.
• Applications:FISH[1, 2]SNP-analysis[1]Nick-translation[2]TUNEL[3]
• Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation:50% Biotin-11-dCTP/ 50% dCTP
• Synonym:γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2’-deoxy-cytidine-5’-triphosphate, Triethylammonium Salt
• Molecular Formula:C28H44N7O16P3S
• Molecular Weight:859.67
• Appearance:Supplied as a liquid in 10mM Tris-HCl, pH 7.5. Labeled with Biotin.
• Purity (HPLC):≥95%
• Concentration:~5mM
• Spectroscopic Properties:λmax 294 nm; ε 9,300 M-1cm-1, Tris-HCl, pH 7.5
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
• Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.